Chromatography Techniques

Chromatography-HPLC in Pharmaceutical

Chromatography and spectroscopy are orthogonal techniques, i.e. their types of information are very different and are specific. Chromatography is a separation method and spectroscopy is a technique which yields a ‘fingerprint’ of individual or from mixture of molecules. HPLC is a technique for separation, quantification and identification of components in a mixture. It is especially suitable for compounds which are not easily volatilized, thermally unstable and have high molecular weights. The advantage of UV method over HPLC method is that the UV method does not require the elaborate treatment and procedures usually associated with chromatographic method. It is less time consuming and economical. The HPLC and UV spectrometry methods are adequate methods to quantify a drug in pure form and its dosage form. Since these methods are simple, specific, rapid, precise and accurate, they may be successfully and conveniently adopted for routine quality control analysis of drugs in bulk and pharmaceutical dosage form.