Chromatography Techniques

Chromatography as Separation Technique

Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis.  Four separation techniques based on molecular characteristics and interaction type use mechanisms of ion exchange, surface adsorption, partition, and size exclusion. Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography. Chromatographic separation methods include adsorption chromatography, ion exchange chromatography, affinity chromatography, and size exclusion chromatography.