Chromatography-HPLC as Separation Techniques
High Performance Liquid Chromatography (HPLC) is a non-destructive procedure for resolving a complex mixture into its individual fractions or compounds. It is based on differential migration of solutes with the solvents. The solutes in a mobile phase go through a stationary phase. Those solutes with a high affinity for the mobile phase will spend more time in this phase than the solutes that prefer the stationary phase. As the solute rise up through the stationary phase they separate. The process is called chromatographic development. The fraction with greater affinity to stationary layer travels slower and shorter distance while that with less affinity travels faster and longer.
- normal-phase chromatography
- Flash Column Chromatography
- Ion exchange chromatography
- Affinity chromatography
- Chiral chromatography
- Size-exclusion chromatography
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