Chromatography Techniques

High Efficiency and High Resolution Techniques

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UHPLC (Ultra-HPLC) or UPLC (Ultra Performance Liquid Chromatography) is now being adopted in industrial labs, especially the pharmaceutical industry due to its high speed, high resolution and solvent saving.  A UHPLC method uses a sub-2micron column as it reduces the analysis time by 80% and save the mobile phase consumption by a huge amount compared to the conventional HPLC. In addition, the much shorter run time significantly reduces UHPLC method development scouting time. HPLC method development principles can be applied to UHPLC method development. Micro and Nano HPLC ensure high levels of flow rate flexibility and reproducibility.

Hydrophilic interaction chromatography or hydrophilic interaction liquid chromatography (HILIC) is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. It uses hydrophilic stationary phases with reversed-phase type eluents.