Day 1 :
- Major Chromatographic Techniques
Location: Webinar
Session Introduction
VÃctor Cerdà ,
University of the Balearic Islands, Spain
Title: Hyphenation of lab-in-syringe with chromatographic techniques
Biography:
Víctor Cerdà, born in Palma de Mallorca, Spain. Graduated and PhD in Chemistry by the University of Barcelona. Lecturer of several universities: Barcelona, Tarragona, Valladolid. Full Professor of Analytical Chemistry in 1982 at the University of the Balearic Islands (UIB). He has conducted 41 Ph.D. Thesis, written 14 books, and has collaborated with 14 chapters in other scientific books. He has published 620 papers and presented 847 contributions in national and international symposia. Chief Researcher of projects funded by the CAICyT, DGICyT, CICyT, BCR (EU), ALFA (EU), AECI, MCyT, and conducted a number of contracts usually related with environmental subjects with different firms (TIRME, MAC INSULAR, ENDESA, etc) and institutions (BCR, Nuclear Security Council, etc). His main research lines is related with the development of new automatic methods in Analytical Chemistry and their application to environmental and pharmaceutical samples. Has been organizer and chairman of more than 15 international symposia. Has been the Head of the Department of Analytical Chemistry and the first Head of the Department of Chemistry of the UIB. Has been Vice-President of the Spanish Society of Analytical Chemistry.
Abstract:
The lab-in-syringe (LIS) is a new technique which has proven to be a very powerful for pre-concentration, cleaning and elimination of interferences in the determination of very different types of organic and inorganic compounds. It can be applied in liquid-liquid or liquid-solid extraction with good results. Its coupling with big instrumentation is especially interesting, such as chromatographic techniques (HPLC, Multi-syringe chromatography, CG and CG/MS) and atomic spectroscopy (graphic camera, ICP_OES, ICP-MS, atomic fluorescence), which provides a great selectivity and sensitivity in the determinations
David Sýkora,
University of Chemistry and Technology Prague, Czech Republic
Title: New HPLC stationary phases prepared by ugi multicomponent synthesis
Biography:
Assoc. Prof. David Sýkora Ph.D. was born at MÄ›stec Králové, Czech Republic. He graduated from the Charles University in Prague, Department of Organic Chemistry. After graduation he worked as an application specialist at Tessek Ltd. where he was responsible for the design and evaluation of new sorbents for HPLC and the development of new chromatographic applications. He received his Ph.D. from the Institute of Chemical Technology (now the University of Chemistry and Technology, UCT Prague). As a postdoctoral fellow, he stayed in the laboratory of Professor J. M. J. Fréchet at the University of California, Berkeley, where he worked in the area of chromatographic characterization of synthetic polymers and development of innovative stationary phases, particularly monoliths, for HPLC, CEC, and GC. Currently he is an Associate Professor at UCT Prague. His interests cover the development of chromatographic methods, mainly suitable for medicinal research and analysis, and the development of new separation media for HPLC, CE, and CEC. His scientific interests also include hyphenated techniques, particularly LC-MS. DS published more than 100 papers in impacted journals.
Abstract:
The column, the ‘heart’ of the HPLC system, has changed greatly with the development of liquid chromatography. The stationary phases packed in the columns have been improved to provide greater separation speed and efficiency, as well as increased stability and reproducibility. Many new sorbents have been introduced for the extension of HPLC/UHPLC to a wider range of sample types or for better separation of compounds, which have in the past proved problematic. However, there exists still vital interest in new stationary phases possessing different separation selectivity or providing an improved separation with respect to commercially available columns. In addition, new techniques of stationary phase synthesis are continuously searched and proposed for their potential to provide novel stationary phases more easily or with some other benefit. In our work, eight different stationary phases based on two aminopropyl silicas of different brands suitable for multimodal chromatography applications have been prepared by a four-component Ugi reaction. Our intention was to synthesize stationary phases significantly differing in their properties, thereby demonstrating flexibility of the Ugi synthetic protocol. Diverse functional groups including a nonpolar long aliphatic chain, a phenyl moiety, a cholic acid scaffold, phenylboronic and monosaccharide units, charged betaine, and arginine moieties were immobilized on a silica surface. The novel sorbents were extensively characterized by a wide variety of analytical techniques, including elemental analysis, Raman spectroscopy, and chromatography. Considering the anchored chemical structures covalently bonded to the silica surface, reversed-phase, hydrophilic, and ion-exchange separation modes were expected. The chromatographic evaluation was performed to map the potential of the individual columns specifically in the mentioned chromatographic modes.
Monika Renuka Sanotra,
Taipei Veteran General Hospital, Taiwan
Title: Gastrocnemius and soleus skeletal muscle proteomic changes of Type 2 diabetes mellitus (T2DM) after exercise interventions in db/db mice
Biography:
Monika Renuka Sanotra,Master in pharmaceutical chemistry from Jamia hamdard India. PhD from from Taipei medical University.Post-doctoral at TVGH. Working experience Drug regulatory affairs and drugs testing at Indian Fda laboratory. worker with tosoh bioscience as application scientist for HPLC column troubleshooting. Major disease biomarkers.
Abstract:
Exercise is a modifiable risk factor that may affect the gastrocnemius and soleus muscle protein level in the T2DM mice model. To investigate this hypothesis, we performed blood examination and proteomics analysis of the skeletal muscle of wild-type mice and diabetic mice with and without exercise training. Inclusion criteria for control and diabetic mice were four male ICR mice per group aged 8 weeks. The three groups consist of 1) wild type (WT), 2) sedentary control diabetic mice (SED) without exercise, and 3) diabetic mice with exercise training (ET) for six weeks. Body weight, HbA1c level before and after exercise intervention, weekly blood glucose monitoring, fatigue test (forelimb grip strength), organs or tissue weight, and biochemical tests. Gastrocnemius and soleus muscles were further used for proteomic analysis by using liquid chromatography and tandem mass spectrometry after tryptic digestion. Proteomic data interpretation was performed by using Peaks7 software and bioinformatics tools.
Biography:
I am Jyotsna Jaiswal ,prsuing a PhD in Biochemistry from University of Allahabad, India. I work under Prof. B Sharma and studied exploration of the plant based principals as Anti-HIV agents and other infectious agents. During the course of research, I have learnt to prepare plant extracts in different solvents, phytochemical characterization assays of these extracts, ananlysis of their free radical quenching abilities by DPPH assay, spectrophotometric analysis of different phytochemicals present in the extracts, assessment of their antioxidative potential in xenobiotics intoxicated rats, and evaluation of their antimicrobial and antiHIV activities. My research interest are virology , pharmacology and microbiology.
Abstract:
Plants hold immense capacity to cure serious illness because of the presence of various phytochemicals with strong medicinal properties. Identification of novel therapeutic agents as new drugs for alleviation of the human suffering from viral diseases is of prime concern. The presence of various phytochemicals in the plant extracts of P. hysterophorus and Argemone mexicana in the leaves, flowers and fruits were analyzed by using thin layer chromatography (TLC) technique. The colored spots present in TLC plates corresponded to different phytochemicals present in the extracts. Liquid Chromatography-Mass Spectrometry (LC-MS) is a technique involved in the separation of delicate and complex mixture, whose constituents are not fully known. The extracts of P. hysterophorus extract prepared in aqueous medium at room temperature displayed the presence of various compounds having different molecular mass. The LCMS analysis demonstrated the presence of 28 different compounds including 1,3 Azulenedicarbonitrile, 2-amino-6-bromo, Selenolo[2,3-b]pyridine-2-carboxylic acid, 3-hydroxy-, ethyl ester, Selenolo[2,3-b]pyridine-2-carboxylic acid, ethyl ester, 7-oxide, phenol, 2-nitro-4-[(trifluoromethyl)sulfonyl]-, and 4-Nitrophenyl trifluoromethane sulfonate etc.
Zama Mthembu
University of KwaZulu Natal, South Africa
Title: Detection of cyanotoxins and measurements of microcystins in South African surface waters by High Performance Liquid Chromatography (HPLC)
Biography:
I am currently working for Diageo as a Quality Assurance and Control Technician completed my Master’s degree in Chemistry part time at University of KwaZulu Natal, where I was doing research in detection of cyanotoxins in South African waters and measurement of microcystins using high performance liquid chromatography/ photo diode array.Attending this convention will benefit me with training that will help me with my method development skills to assist in monitoring the risk towards environmental health.
Abstract:
Cyanobacteria, an algae bloom responsible for the creation of deadly toxins. These toxins have the potential to adversely impact human and animal health. Microcystins (MCs), are harmful toxins that are produced by cyanobacteria species and the most investigated toxins worldwide. However, in South Africa their occurrence statistics are deficient due to inaccessibility of reliable, sensitive, and precise analytical methods to investigate them in water surfaces. Thus, a development of analytical methods for the detection and accurate quantification of algal toxins is crucial to consider the health risk of exposure to toxins in wastewater and surface waters. This study recommends an analytical method for the detection and quantification of algal toxins using solid phase extraction (SPE) and high-performance liquid chromatography (HPLC) coupled with photodiode detector (PDA). The developed method was validated based on linearity which ranged from 3 – 2500 µg L-1, recoveries at 99.6 – 113.9%, the limit of detection (LOD) ranging from 0.2 – 480 µg L-1, and limit of quantification (LOQ) at 0.7 – 160 µg L-1 and the regression coefficients obtained were above 0.9880 in all targeted analytes. The developed method was applied to some of KwaZulu Natal surface water and Darville wastewater treatment plant, South Africa. MC-RR was the most detected analyte at concentration ranging from 0.069 ng L-1 – 30.521 ng L-1 compared to other targeted MCs. The developed method is simple and cheap, which can consent developing countries to monitor these deadly MC toxins in water surfaces.