Monika Renuka Sanotra,
Taipei Veteran General Hospital, Taiwan
Title: Gastrocnemius and soleus skeletal muscle proteomic changes of Type 2 diabetes mellitus (T2DM) after exercise interventions in db/db mice
Biography
Biography: Monika Renuka Sanotra,
Abstract
Exercise is a modifiable risk factor that may affect the gastrocnemius and soleus muscle protein level in the T2DM mice model. To investigate this hypothesis, we performed blood examination and proteomics analysis of the skeletal muscle of wild-type mice and diabetic mice with and without exercise training. Inclusion criteria for control and diabetic mice were four male ICR mice per group aged 8 weeks. The three groups consist of 1) wild type (WT), 2) sedentary control diabetic mice (SED) without exercise, and 3) diabetic mice with exercise training (ET) for six weeks. Body weight, HbA1c level before and after exercise intervention, weekly blood glucose monitoring, fatigue test (forelimb grip strength), organs or tissue weight, and biochemical tests. Gastrocnemius and soleus muscles were further used for proteomic analysis by using liquid chromatography and tandem mass spectrometry after tryptic digestion. Proteomic data interpretation was performed by using Peaks7 software and bioinformatics tools.