Jyotsna Jaiswal,
University of Allahabad, India
Title: Chromatographic analysis of phytochemicals from parthenium hysterophorus and argemone mexicana using thin layer chromatography and liquid chromatography- mass spectrophotometry and biological applications
Biography
Biography: Jyotsna Jaiswal,
Abstract
Plants hold immense capacity to cure serious illness because of the presence of various phytochemicals with strong medicinal properties. Identification of novel therapeutic agents as new drugs for alleviation of the human suffering from viral diseases is of prime concern. The presence of various phytochemicals in the plant extracts of P. hysterophorus and Argemone mexicana in the leaves, flowers and fruits were analyzed by using thin layer chromatography (TLC) technique. The colored spots present in TLC plates corresponded to different phytochemicals present in the extracts. Liquid Chromatography-Mass Spectrometry (LC-MS) is a technique involved in the separation of delicate and complex mixture, whose constituents are not fully known. The extracts of P. hysterophorus extract prepared in aqueous medium at room temperature displayed the presence of various compounds having different molecular mass. The LCMS analysis demonstrated the presence of 28 different compounds including 1,3 Azulenedicarbonitrile, 2-amino-6-bromo, Selenolo[2,3-b]pyridine-2-carboxylic acid, 3-hydroxy-, ethyl ester, Selenolo[2,3-b]pyridine-2-carboxylic acid, ethyl ester, 7-oxide, phenol, 2-nitro-4-[(trifluoromethyl)sulfonyl]-, and 4-Nitrophenyl trifluoromethane sulfonate etc.