Dagmar Heinova
University of Veterinary Medicine in Kosice, Slovakia
Title: Isoelectric focusing technique as a tool for separation of bird lactate dehydrogenase isoenzymes
Biography
Biography: Dagmar Heinova
Abstract
Statement of the Problem: Lactate dehydrogenase (EC 1.1.1.27, LDH) is an enzyme ubiquitously distributed in cells of vertebrates, plants, and bacteria. Structurally, it is a tetramer of four units which in animals exists in five electrophoretically distinguishable forms known as isoenzymes. Electrophoretic techniques routinely applied in separating mammalian LDH isoenzymes use a buffer system of pH 8.6 at which their electrophoretic migration depends on the migration of the two pure types, i.e., H4 and M4 forms. The more the two homotetramers differ in charge, the more separable are the hybrids by electrophoresis. In the case of bird LDHs, the two pure types migrate close together towards the anode at pH 8.6 producing only one diffuse enzymatic zone.
Methodology & Theoretical Orientation: For the separation of bird LDHs, we chose isoelectric focusing technique in a gradient of pH 3 to 9. Separation conditions were used as follows: 2000 V, 2.5 mA, 3.5 W, 15ºC, 20 min separation time. Gels were stained with nitro blue tetrazolium technique in 0.1 M Gly-NaCl-NaOH buffer pH 8.3 at 37ºC for 30 min.
Findings: Using above described methodology we achieved good and clear resolution of all five forms of the enzyme of bird origin with their localization being in the pH region of 6.2 to 8.1 (chicken LDH isoenzymes), 5.4 to 7.7 (pheasant LDHs), and 5.3 to 8.3 (turkey lactate dehydrogenase isoenzymes). Mammalian molecules of LDH were more acidic and widespread with the pI values being in the range of 4.5 to 9.0.
Conclusion & Significance: Using IEF technique it was possible to compare the pattern of LDH isoenzymes in serum and tissues of mammals and birds as well as to observe the pattern of the enzymes in some tissues of chicken embryo.