Karen Gaudin
Universityof Bordeaux, France
Title: Study of amphiphile molecules by RP-UHPLC-DAD/CAD for vectorization applications
Biography
Biography: Karen Gaudin
Abstract
The modern pharmaceutical technology is constantly looking for new strategies of drug delivery. One of the most popular concepts is closing the therapeutic compounds in a supra-molecular nano-system formed by amphiphilic molecules. As a consequence, the chemically investigation of nano-objects requires high effective methods for qualitative and quantitative analysis of amphiphiles. Among them UHPLC can be used to characterize amphiphilic molecules and quantifying them in nano-assembly. The most common chromatographic technique first envisaged for this investigation is Reversed Phase Liquid Chromatography (RP-HPLC). It takes into consideration both parts of amphiphiles: the hydrophilic and lipophilic moieties of the molecule. The double detection using Diode Array Detector (DAD) and Charged Aerosol Detector (CAD) was selected because of the lipidic nature of this class of molecule, especially for the compounds without chromophores. This analytical development was performed with three amphiphilic molecules: DOTAU, diC16dt and DOPC which are cationic, anionic and zwitter-ionic molecules, respectively, which are synthetized for biomedical application. Several parameters were studied for their elution: solvent nature, pH, and column. The results contribute to understand behaviour of amphiphile molecules and they encourage to the further exploration this research area. Thanks to this study, purity and stability of these molecules were established in various solvents to support formulation purposes. The final perspectives of this approach will be quantification step during nano assembly achievements.